Protein bead array for the detection of HIV-1 antibodies from fresh plasma and dried-blood-spot specimens.

the observed differences between the two methods. Our study is even more intriguing if we consider that, very recently, a new molecular form of PTH, with structural integrity of the PTH(1– 4) region and a modified PTH(15–20) region, has been identified by HPLC in primary and secondary hyperparathyroidism (21). In fact, this newly discovered form of PTH appears to be immu-noreactive and detectable by the whole-PTH assay but not by the intact-PTH assay. In conclusion, the unexpected constant differences in PTH values among immunoassays, observed in both uremic and PHPT patients and in healthy individuals, could be attributable to the different calibration procedures in addition to the presence of PTH fragments. It would be useful for manufacturers to reduce the systemic variability among methods by use of a more standardized method of calibration and use of antibodies that recognize the only biologically active PTH molecule. Accumulation of a non-(1– 84) molecular form of a parathyroid hormone (PTH) detected by intact PTH assay in renal failure: importance in the interpretation of PTH values. et al. Origin of parathyroid hormone (PTH) fragments detected by intact-PTH assays. non-(1– 84) circulating parathyroid hormone (PTH) fragment interferes significantly with intact PTH commercial assay measurements in uremic samples. New assays for intact parathyroid hormone and their clinical relevance for the diagnosis of hyperparathyroid-ism. Plasma levels of parathyroid hormone (1– 84) whole molecule and parathy-roid hormone (7– 84)-like fragments in pseudohypoparathyroidism type I. Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1– 84: implications for improvement of accurate assessment of parathyroid function. Comparison of intact and " whole molecule " parathyroid hormone assays in patients with histologically confirmed post-renal transplant osteodystrophy.terminal form of parathyroid hormone (PTH) with immunologic similarities to hPTH(1– 84) is overproduced in primary and secondary hyperparathyroidism. Simultaneous multianalyte immunoassays offer advantages , including reduction of technical operations, execution time, and overall costs. The suspension array technology (SAT), which uses microfluorospheres with flow cytometry, is a multianalyte immunoassay that requires small specimen volumes, such as those obtained by less-invasive blood sampling approaches such as heel or finger sticks, as well as those obtained in pediatric specimens. The dried-blood-spot (DBS) technology has become an important screening tool for clinical and epidemiologic testing (1–3). It is particularly convenient in rural, resource limited settings where trained personnel and adequate facilities for blood collection, processing, transport, and storage may not be available. This approach, …